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Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

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1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....

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Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....

Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....

Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....

Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....

Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....

Introduction For almost a century various steroid hormone determinations have been performed from a variety of body fluids in the diagnostic laboratory to gather relevant information and assist clinicians in the diagnosis of various pathological conditions. Perhaps the most notable activity occurred in the 1960’s when new analytical methods emerged that demonstrated superior analytical sensitivity at the low end of expected concentration ranges. In the following discussion, we describe various analytical procedures for the determination of steroid hormones, which have a characteristic molecular structure as shown on the title page of this pamphlet. The structure consists of 3 hexagons and one pentagon linked together in classical biochemical configuration. Following is a list of the more prominent steroid hormones. ....

1. In order to achieve proper saliva sampling special care must be taken to the extremely low concentrations of the analytes under investigation. We are recommending the use of special polypropylene devices like the ones enclosed in this Saliva Set. Such sampling sets are available from Demeditec Diagnostics. These sampling devices have very low absorption characteristics and are easy to handle also for non-medical individuals. 2. Ovulating women should collect saliva at best during day 21 of their cycle (first day of monthly bleeding = day 1). In case of hormone replacement therapy by application of creams the patient must clean the hands carefully after each application. In order to avoid any possible contamination these patients also must wear thin plastic gloves (single use) before starting the collection procedure. ....